Luminex 100 IS Version 2.1 - Manual (Page 23)

x MAP Technology Luminex xMAP Technology
PN 89-00002-00-062 Rev. A 17
Figure 7. Set DD Gate Example
‹ To determine optimal gate settings:
1. Create a New Advanced batch with DD gate settings at 0 - 32767
(make sure all other settings are appropriate for your sample).
2. On plate layout choose a single well with the Acquire command
to obtain a single sample.
3. Click and drag the left red line to a position on the left side of the
major peak to eliminate small particles.
4. Click and drag the right red line to a position on the right side of
the major peak to eliminate aggregate beads.
Note: The gate that is in effect
when collecting data determines
which values are used in the
result. Applying a gate or
changing a gate for existing data
does not change your calculated
values. The gate positions used
while collecting data are the
numerical values selected in the
template or the New Advanced
Batch.
Gate
boundaries
Numerical gate position (current values)
Aggregate
Beads
Table 5: DD Gate Relationships
Gate Width:
Wider Narrower
%CV (within sample) Higher Lower
Sample Acquisition
Speed Faster Slower
Note: The sample is
representative of the expected
assay samples. Gate positions
are dependent upon buffer
composition. Any changes
made to your assay buffer
composition may result in a
different optimal gate
location.