Bio-Rad SsoAdvanced™ Universal SYBR® Green Supermix - Manual (Page 4)

SsoAdvanced ™ Universal SYBR ® Green Supermix Instruction Manual ii | ii |
SsoAdvanced ™ Universal SYBR ® Green Supermix
Catalog # Supermix Volume Kit Size
172-5270 2 ml (2 x 1 ml vials) 200 x 20 μl reactions
172-5271 5 ml (5 x 1 ml vials) 500 x 20 μl reactions
172-5272 10 ml (10 x 1 ml vials) 1,000 x 20 μl reactions
172-5274 25 ml (5 x 5 ml vials) 2,500 x 20 μl reactions
172-5275 50 ml (10 x 5 ml vials) 5,000 x 20 µl reactions
Shipping and Storage
The SsoAdvanced universal SYBR ® Green supermix is shipped on dry ice. Upon receipt, the
supermix should be stored at –20ºC in a constant temperature freezer and protected from
light. When stored in these conditions, the supermix is guaranteed for one year. When stored
at 4ºC, the supermix is guaranteed for three months. To avoid excess freeze-thaw cycles, we
recommend preparing aliquots for storage.
Kit Contents
SsoAdvanced universal SYBR ® Green supermix is a 2x concentrated, ready-to-use reaction
master mix optimized for dye-based real-time PCR on any real-time PCR instrument (ROX-
independent and ROX-dependent). It contains antibody-mediated hot-start Sso7d fusion
polymerase, dNTPs, MgCl2, SYBR
® Green I dye, enhancers, stabilizers, and a blend of passive
reference dyes (including ROX and fluorescein).
Instrument Compatibility
This supermix is compatible with all Bio-Rad and ROX-dependent Applied Biosystems real-time
PCR instruments, and with the Roche LightCycler LC480, Qiagen Rotor-Gene Q, Eppendorf
Mastercycler ep realplex, and Stratagene Mx real-time PCR systems.
Product Use Limitations
The SsoAdvanced universal SYBR ® Green supermix is intended for research use only, and is
not intended for clinical or diagnostic use.
Technical Assistance
Bio-Rad Laboratories takes great pride in providing best-in-class technical support through
our online, telephone, and field support. To obtain support, please visit
www.bio-rad.com , call
1.800.4.BIORAD, or contact your local field applications scientist.
Quality Control
SsoAdvanced universal SYBR ® Green supermix demonstrates high PCR efficiency and linear
resolution over a wide linear dynamic range. Stringent specifications are maintained to ensure
lot-to-lot consistency. This product is free of detectable DNase and RNase activities.