Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 15)

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Both gel types gels are available in polyacrylamide single percentages and gradients. Use the protein
migration charts and tables to select the gel type that optimizes resolution of your sample:
n
Use single-percentage gels to separate bands of similar molecular weight. Optimum separation
occurs in the lower half of the gel, so use a percentage in which the protein migrates to the
lower half of the gel
n
Use gradient gels to separate samples containing a broad range of molecular weights. Gradient
gels allow resolution of both high- and low-molecular weight bands on the same gel. Larger
pore sizes at the top of the gel permit resolution of larger molecules, smaller pore sizes toward
the bottom of the gel restrict excessive separation of small molecules
Gel Composition
Crosslinker 2.6% C
Stacking gel 4% T, 2.6% C
Shelf life ~12 months at 2–8°C; expiration date is printed on package
Gel Percentage Optimum Separation Gel Percentage Optimum Separation
Range Range
7.5% 40–200 kD 4–15% 20–250 kD
10% 30–150 kD 4–20% 10–200 kD
12% 20–120 kD Any kD ™ 10–100 kD
Migration charts for protein standards on Mini-PROTEAN TGX and Mini-PROTEAN TGX Stain-Free gels.
Mini-PROTEAN ® TGX ™ Precast Gels
Any kD ™ 4–20% 4–15% 12% 10% 7.5%
Broad Range Unstained
200
116
97.4
66
45
31
21.5
14.4
6.5
200
116
97.4
66
45
31
21.5
14.4
6.5
200
116
97.4
66
45
31
21.5
14.4
6.5
200
116
97.4
66
45
31
21.5
14.4
200
116
97.4
66
45
31
21.5
200
116
97.4
66
45
31
Any kD ™ 4–20% 4–15% 12% 10% 7.5%
Precision Plus Protein ™ Unstained
250
150
100
75
50
37
25
20
15
10
250
150
100
75
50
37
25
20
15
10
250
150
100
75
50
37
25
20
15
10
250
150
100
75
50
37
25
20
15
250
150
100
75
50
37
25
20
250
150
100
75
50
37
Instruction Manual and Application Guide