Iris Sample Processing StatSpin® CytoFuge 2 Compact Cytocentrifuge System - Manual (Page 10)

6
Access the Rotor
Access the rotor cover by turning the knurled knob 3 (cover nut) counterclockwise while holding the
rotor itself to prevent turning. (See Figure 1.)
Remove the Rotor
1. Hold the rotor6 with one hand and remove the rotor cover5.
2. Remove the three-sided nut2 by turning it counterclockwise.
3. Lift the rotor6 straight out from the centrifuge bowl.
Replace the Rotor
1. Place the rotor6 on the two rotor indexing pins, aligning the corresponding holes.
2. Tighten the rotor hold-down nut2 by turning it clockwise.
3. Replace the rotor cover5.
CAUTION - Be careful when replacing the rotor. Make sure the two locator pins are in
the corresponding holes in the rotor. Do not over tighten the hold-down nut. Make
certain it is replaced with the flat surface facing up.
CAUTION - NEVER use any tool to tighten either the rotor nut or the cover nut.
Add Sample to the Cell and Filter Concentrator
Place assembled Cell and Filter Concentrators in the CytoFuge rotor before loading sample.
Process the specimen as quickly as possible following sample addition to prevent cells from settling.
Slowly add sample to the bottom of the funnel. Avoid getting droplets onto the walls of the funnel.
Avoid exposing the filter material or the microscope slide to sample during the loading process. Do
not overfill the device.
NOTE: In a properly loaded Concentrator, while in the “rest” position, the fluid does not contact the slide.
During the first few rotations the Concentrators tilt to a vertical orientation, (noted by an audible
click) bringing the fluid in contact with the microscope slide. The Concentrators remain upright
until rotation has nearly stopped at which time they shift back to the rest position
.
1. Rotor Gasket
2. Rotor Hold-Down Nut
(3-lobed)
3. Cover Nut
4. Cell Concentrator
(shown in rest position)
5. Rotor Cover
6. Rotor
7. Bowl Gasket
8. CytoFuge Bowl
9. Motor
10. Rotor Mount
11. Rotor Indexing Pin
Figure 1. Cross section of the CytoFuge 2 showing the bowl and rotor with the names
and locations of the parts. The rotor has four positions, allowing two or four
concentrators to be centrifuged.