ANKOM TDF Dietary - Manual (Page 17)

Operator’s Manual
Rev E 12/2/14
pg. 17
IDF/SDF Analysis
An IDF/SDF analysis measures the amount of IDF and SDF within a given sample. This requires the use of ANKOM DF-I and
DF-S filter bags. When starting a new run, the ANKOM
TDF
Dietary Fiber Analyzer must have bags installed at all stations (a
total of twelve bags).
To enhance the productivity of your instrument, you can begin the IDF process of a
new run while the SDF process of a previous run is finishing. See the “Productivity
Enhancement” section of this manual for more details.
To perform an IDF/SDF analysis, follow the steps below.
1. Prepare chemicals and enzymes.
When using the ANKOM TDF Dietary Fiber Analyzer for the AOAC 991.43, AACC 32-07.01, and NMKL 129,2003
methods use the chemicals and enzymes referred to therein. See Appendix A of this manual for the list of chemicals and
enzymes and the instructions for how to prepare them for use in this instrument.
2. De-fat (see "Appendix C – De-fatting Procedure" in this manual) and de-sugar your samples as
needed according to the AOAC 991.43, AACC 32-07.01, or NMKL 129,2003 methods.
3. Label the filter bags using a Solvent Resistant Marker.
4. Prepare for data collection.
You will need a place to store the data collected during this analysis. For your convenience a CD titled “Calculation
Templates” was included with the instrument. This CD has data spreadsheets that can be used for this analysis. Please
read the “Instructions” tab in the MS-Excel file for information about the spreadsheets.
5. Weigh filter bags.
Using a Bag Weigh Holder is critical to eliminate the effects of static electricity
during the weighing process.
Roll or fold each bag and place it in a tared Bag Weigh Holder. Place the Bag Weigh Holder in the center of a balance
and record the weight.
Because different balances have different sensitivities, the Bag Weigh Holder
should be placed in the center of the balance for best results.
IDF Bag (shorter bag)
SDF Bag (longer bag)