Eppendorf BioPhotometer plus - Manual (Page 42)

10 Technical data
42
BioPhotometer plus — Operating manual
10.7 Application parameters
Method memory 32 preprogrammed, modifiable method
programs
Measuring methods End point against blank
Dye methods: parallel measurement of
biomolecule and dye label
Method-dependent evaluation absorbance
concentration via factor
concentration via calibration with 1 to 10
standards:
• single point calibration (1 standard)
• linear regression (2 to 10 standards)
• non-linear regression (polynome of 3rd
degree; 4 or 5 to 10 standards 
(see Evaluation procedure on page 43)).
• 1x, 2x or 3x measurement
for nucleic acids:
• ratio 260/280
• ratio 260/230
• molar concentration
• total yield
for dye methods:
• FOI (frequency of incorporation; 
marking density)
Calibration memory For all calibration methods
Measured value memory For 100 results with abosrbance and ratio
values, sample number, sample dilution, date
and time
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