Eppendorf twin.tec PCR Plates - Manual (Page 2)

Instructions for use
Eppendorf twin.tec
® PCR Plates
English (EN)
2
 Take appropriate measures to avoid contaminations after opening the bag containing
PCR clean consumables.
 Reagents, consumables contaminated with reagents and materials used for cleaning
and disinfecting should be disposed of in accordance with laboratory regulations.
3 Application
For PCR instructions please refer to reagent manufacturers' work specifications or lab
protocols.
3.1 Filling
Maximum filling volume:
• twin.tec PCR Plate 96, semi-skirted and unskirted: 250 µL
• twin.tec PCR Plate 96, skirted and unskirted, low profile: 150 µL
• twin.tec PCR Plate 384: 45 µL
• twin.tec
real-time PCR Plate 384: 40 µL
 Fill the twin.tec PCR Plates directly or place them in an appropriate stand for filling.
3.2 Sealing
 You can use cap strips, adhesive PCR foils and films or heat sealing foils and films to
seal the twin.tec PCR Plates.
We recommend heat sealing with Eppendorf Heat Sealing Foil or Eppendorf Heat
Sealing Film as the ideal protection against evaporation.
Perfect sealing of the PCR Plates allows an oil-free PCR reaction in a minimum volume of
up to 10 µL (96-well PCR Plates) or 5 µL (384-well PCR Plate).
3.3 Real-time PCR
An application for plates with white wells is to enhance the signal recovery and thus the
detection limit in real-time PCR applications. This is the case with a dye which has a low
concentration or low fluorescence intensity. Generally plates with white, signal amplifying
wells can cause irradiation - depending on the device - if the concentration of the dye is
too high. In this case, you should reduce the concentration of the probe or of the dye.