22 conditioning – Metrohm 940 Professional IC Vario ONE/ChS/PP/LPG User Manual

Page 45

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4 Installation

940 Professional IC Vario ONE/ChS/PP/LPG (2.940.1350)

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37

Rinse the separation column with eluent for approximately 10
minutes.

Use manual control in MagIC Net to stop the high-pressure pump
again: Off.

4.22

Conditioning

After installation, after each time the instrument is switched on and after
each time the eluent is changed, the system has to be conditioned with
eluent long enough that a stable baseline is attained.

NOTE

The conditioning time can lengthen considerably after changing the
eluent.

Conditioning the system

1 Preparing the software

CAUTION

Ensure that the configured flow is not higher than the flow permit-
ted for the corresponding column (refer to the column leaflet and
the chip data record).

Start the MagIC Net computer program.

Open the Equilibration tab in MagIC Net: Work-
place

Run Equilibration.

Select (or create) a suitable method.
Also see: MagIC Net tutorial and online help.

2 Preparing the instrument

Ensure that the column is inserted correctly in relation to the flow
direction marked on the sticker (arrow has to point in the direc-
tion of flow).

Ensure that the eluent aspiration tubing is immersed in the eluent
and there is enough eluent in the eluent bottle.

3 Starting equilibration

Start the equilibration in MagIC Net: Workplace

Run Equi-

libration

Start HW.

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