Bio-Rad SingleShot™ Cell Lysis RT-qPCR Kits - Manual

Introduction
The SingleShot Cell Lysis Kit prepares genomic DNA (gDNA)–free RNA directly from
cell culture in approximately 20 min for use in reverse transcription quantitative
PCR (RT-qPCR) applications. This kit is compatible with an input of 100,000–10 cells
from suspension, adherent cells, or primary cells from cell cultures. With the SingleShot
Cell Lysis Kit, gene expression analysis can be completed in approximately 2 hours
from cell culture to quantification cycle (Cq) using either a two-step or a one-step
RT-qPCR workflow.
Kit Contents
Cell Lysis Reagents 100 x 50 μl reactions 500 x 50 μl reactions
SingleShot Cell Lysis Buffer 5 ml (1 x 5 ml vial) 25 ml (5 x 5 ml vial)
Proteinase K Solution 100 µl (1 x 1 ml vial) 500 µl (1 x 1 ml vial)
DNase Solution 100 µl (1 x 1 ml vial) 500 µl (1 x 1 ml vial)
Store all components at –20°C for up to 1 year.
RT-qPCR Products Required but Not Provided
The SingleShot Cell Lysis Kit is validated for the products listed in Table 1.
Additional Reagents Required but Not Provided
■ ■
Phosphate buffered saline (PBS) for washing the cells
■ ■
TE buffer (nuclease-free) pH 7.5 for use with the PrimePCR Reverse Transcription
Control Assay
Table 1. Validated RT-qPCR products for use with SingleShot Cell Lysis Kit.
Catalog # Description
Two-Step RT-qPCR Products
172-5038
iScript ™ Advanced cDNA Synthesis Kit for RT-qPCR, 100 reactions
172-5271 SsoAdvanced ™ Universal SYBR ® Green Supermix, 500 reactions
172-5281 SsoAdvanced Universal Probes Supermix, 500 reactions
One-Step RT-qPCR Products
172-5151
iTaq ™ Universal SYBR ® Green One-Step Kit, 100 reactions
172-5141 iTaq ™ Universal Probes One-Step Kit, 100 reactions
Control (optional)
Online order only
PrimePCR ™ Reverse Transcription Control Assay (bio-rad.com/primepcr)
For research purposes only.
Catalog # Description
172-5080 SingleShot Cell Lysis Kit, 100 x 50 µl reactions
172-5081 SingleShot Cell Lysis Kit, 500 x 50 µl reactions
SingleShot
™
Cell Lysis Kit