Bio-Rad Mini-PROTEAN® Tetra Cell User Manual

Page 31

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Section 5

References

Laemmli UK (1970). Cleavage of structural proteins during the

assembly of the head of bacteriophage t4. Nature 227, 680-685.

Ornstein L (1964). Disc electrophoresis. I. Background and theory.

Ann N Y Acad Sci 121, 321-349.

Chrambach (1983). A and Jovin, T M, Electrophoresis, 4, 190–204

McLellan T (1982). Electrophoresis buffers for polyacrylamide gels

at various ph. Anal Biochem 126, 94-99.

Section 6

Maintenance

Mini-PROTEAN Tetra tank and lid,

Rinse thoroughly with distilled water

electrode assembly, companion after

every use. assembly, casting stand,

and frame

Rinse thoroughly with distilled water

electrode after every use

Glass plates and combs

Wash with a laboratory detergent, then

rinse thoroughly with distilled water.

Limit submersion of spacer plates

in strongly basic solutions, such as

>100 mM NaOH, to less than 24 hr.

Limit submersion in chromic-sulfuric

acid glass cleaning solution to 2–3 hr.

Prolonged submersion compromises

the integrity of the adhesive.

To preserve the longevity of the

adhesive bond, avoid extended

submersion (>5 days) in cleaning

solution made from Bio-Rad cleaning

concentrate (catalog #161-0722) or

other strongly basic detergents.

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