Bio-Rad PROTEAN® i12™ IEF System User Manual
Page 52
Appendix C IEF Protocols
PROTEAN i12 IEF System Instruction Manual
48 |
48 |
Step Voltage (V)
Ramp
Time
Units
1
10,000
Rapid
43,000
Volt Hr
2
1,000
Hold
Step Voltage (V)
Ramp
Time
Units
1
250
Rapid
0:30
HH:MM
2
10,000
Gradual
2:00
HH:MM
3
10,000
Rapid
43,000
Volt Hr
4
1,000
Hold
17cm pH5.5–6.7
17cm pH6.3–8.3
17cm pH7–10 R
17cm pH7–10 G
17cm pH3–10 R
17cm pH3–10 NL R
17cm pH4–7 R
17cm pH5–8 R
17cm pH3–10 G
17cm pH3–10 NL G
17cm pH4–7 G
17cm pH5–8 G
17cm pH3–6 R
17cm pH3.9–5.1
17cm pH4.7–5.9
18cm pH3–10 R
18cm pH3–10 NL R
18cm pH4–7 R
18cm pH5–8 R
18cm pH3–10 G
18cm pH3–10 NL G
18cm pH4–7 G
18cm pH5–8 G
18cm pH3–6 R
17cm pH3–6 G
18cm pH3–6 G
Step Voltage (V)
Ramp
Time
Units
1
10,000
Rapid
32,000
Volt Hr
2
1,000
Hold
Step Voltage (V)
Ramp
Time
Units
1
250
Rapid
0:30
HH:MM
2
10,000
Gradual
2:00
HH:MM
3
10,000
Rapid
32,000
Volt Hr
4
1,000
Hold
18cm pH3.9–5.1
18cm pH4.7–5.9
18cm pH5.5–6.7
18cm pH6.3–8.3
18cm pH7–10 R
18cm pH7–10 G
Step Voltage (V)
Ramp
Time
Units
1
250
Rapid
0:30
HH:MM
2
10,000
Gradual
2:00
HH:MM
3
10,000
Rapid
50,000
Volt Hr
4
1,000
Hold
Step Voltage (V)
Ramp
Time
Units
1
250
Rapid
0:30
HH:MM
2
10,000
Gradual
2:00
HH:MM
3
10,000
Rapid
63,000
Volt Hr
4
1,000
Hold
Step Voltage (V)
Ramp
Time
Units
1
10,000
Rapid
46,000
Volt Hr
2
1,000
Hold
Step Voltage (V)
Ramp
Time
Units
1
250
Rapid
0:30
HH:MM
2
10,000
Gradual
2:00
HH:MM
3
10,000
Rapid
46,000
Volt Hr
4
1,000
Hold