Pressure stability, Temperature stability, Sterilization – Bio-Rad Glass Econo-Column® Accessories User Manual
Page 5: Column cleaning, Accessories, Flow adaptors
Econo-Column
Flow Adaptor
Reagent
20°C
50°C
20°C
50°C
Citric acid
E
E
E
E
Cresol
G
F
G
F
Cyclohexane
F
N
F
N
Cyclohexanone
F
N
F
N
Dimethyl formamide
N
N
N
N
Dimethylsulfoxide
-
-
-
-
Ethyl acetate
N
N
N
N
Ethyl alcohol
E
G
E
G
Ethyl benzene
-
-
-
-
Ethyl chloride, liquid
F
N
F
N
Formaldehyde, 40%
E
G
E
G
Formic acid, 98–100%
E
G
E
G
Glycerine
E
E
E
E
Hydrochloric acid, 35%
E
G
E
G
Hydrofluoric acid, 48%
E
E
E
E
Hydrogen peroxide, 90%
E
-
E
-
Isobutyl alcohol
E
E
E
E
Isopropyl alcohol
E
E
E
E
Lactic acid, 85%
G
F
G
F
Methylene chloride
N
N
N
N
Nitric acid, 10%
E
E
E
E
Nitric acid, 50%
F
N
F
N
Nitrobenzene
N
N
N
N
Perchloric acid
G
N
G
N
Phenol, liquid
N
N
N
N
Phosphoric acid, 5%
E
E
E
E
Phosphoric acid, 85%
E
G
E
G
Propionic acid
E
G
E
G
Sodium acetate, saturated
E
E
E
E
Sodium hydroxide, 1%
E
E
E
E
Sodium hydroxide, 50% to saturated
E
G
E
G
Sodium hypochlorite
G
F
G
F
Sulfuric acid, 6%
E
E
E
E
Sulfuric acid, 60%
E
G
E
G
Sulfuric acid, 98%
F
N
F
N
Toluene
G
F
G
F
Trichloroacetic acid
F
N
F
N
Trichloroethane
N
N
N
N
Trichloroethylene
N
N
N
N
Tris buffer, solution
E
G
E
G
Urea
E
E
E
E
Xylene
F
N
F
N
Reprinted by permission of Nalge Company
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Pressure Stability
Econo-Column chromatography columns are designed for low-pressure applica-
tions, i.e., less than 1.0 atm. Use caution when operating the column with a
pump. A disruption in flow may cause a rapid rise in backpressure. If this occurs,
immediately shut off the pump and check the gel bed for compression.
Temperature Stability
Econo-Column chromatography columns and flow adaptors can be operated at
temperatures up to 50°C. For applications requiring temperature control, jacket-
ed columns are available in various sizes and formats. Refer to the Bio-Rad Life
Sciences Research Products catalog for more information.
Sterilization
Econo-Column chromatography columns and accessories can be sterilized with
2 N NaOH or 100% ethanol. The columns can also be autoclaved, wet or dry, at
a maximum of 15 min at 121°C.
Column Cleaning
Econo-Column chromatography columns can be cleaned with 70% ethanol. The
bed support pore size is 20 µm and may require intermittent cleaning. To clean
the bed support, backwash with 1 N NaOH.
Accessories
A full range of Econo-Column accessories are also available. For more detailed
ordering information, refer to the Bio-Rad Life Science Research Products cata-
log.
Flow Adaptors
Flow adaptors improve resolution and reduce mechanical disruption by deliver-
ing sample and buffer directly to the top of the gel bed.
Catalog#
Description
738-0014
Flow Adaptor, 1 cm ID, 7 cm long
738-0015
Flow Adaptor, 1 cm ID, 14 cm long
738-0016
Flow Adaptor, 1.5 cm ID, 14 cm long
738-0017
Flow Adaptor, 2.5 cm ID, 14 cm long
738-0018
Flow Adaptor, 5.0 cm ID, 14 cm long
738-0019
Econo-Pac
®
Flow Adaptor, 1.5 cm ID, 7 cm long
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