Bio-Rad PureZOL™ RNA Isolation Reagent User Manual
Page 16
Problem
Possible Cause
Recommended Solution
Low yield
Insufficient amount of
Scale up the amount of
(continued)
PureZOL used for
PureZOL used according to
sample lysis and
sample size. Sample volume
homogenization
should not exceed 10% of
the PureZOL reagent used
for homogenization
Lysate was not
Make sure to incubate the
incubated at room
lysate after the disruption
temperature for
step for 5 minutes at room
5 minutes after the
temperature to allow
disruption step (see
complete dissociation of
step 4 in protocol)
nucleoprotein complexes
RNA is
RNase contamination
Treat all user-made solutions
degraded
of solutions supplied
with DEPC before use (see
by the user
section 3, Maintaining an
RNase-Free Environment)
RNase contamination
See section 3, Maintaining
of plasticware and
an RNase-Free Environment
work station
Frozen tissue samples
Add PureZOL directly to
were allowed to thaw
frozen samples before they
or sit at room
thaw. Do not let starting
temperature
samples sit at room
temperature
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