Metrohm 940 Professional IC Vario ONE/Prep 1 - Manual (Page 36)

4.19 Connecting the separation column
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940 Professional IC Vario ONE/Prep 1 (2.940.1110)
￭ Start manual control in MagIC Net and select the high-pressure
pump: Manual
▶ Manual control ▶ Pump
– Flow: in accordance with column leaflet
– On
￭ Rinse the guard column with eluent for approx. 5 minutes.
￭ Use manual control in MagIC Net to stop the high-pressure pump
again: Off.
4.19 Connecting the separation column
CAUTION
New separation columns are filled with solution and sealed with stop-
pers on both sides. Before inserting the column, ensure that this solu-
tion can be mixed with the eluent being used (follow information from
the manufacturer).
NOTE
The separation column may not be connected until after the instrument
has already been put into operation once (see Chapter 4.17, page 25).
The guard column and separation column have to be replaced by a
coupling (6.2744.040) until then.