Bio-Rad Bio-Plex System Validation and Calibration Tools User Manual
Page 16
10.3 Validation of Fluidics Integrity
Procedure
1.
If not already done, follow the procedure for start-up and calibration of the
Bio-Plex system.
N
No
otte
e:: B
Be
e s
su
urre
e tto
o c
ca
alliib
brra
atte
e iim
mm
me
ed
diia
atte
elly
y b
be
effo
orre
e v
va
alliid
da
attiio
on
n.. IIff y
yo
ou
u a
arre
e u
us
siin
ng
g B
Biio
o--P
Plle
ex
x
M
Ma
an
na
ag
ge
err 4
4..0
0//4
4..1
1,, u
us
se
e e
eiitth
he
err tth
he
e h
hiig
gh
h o
orr llo
ow
w R
RP
P1
1 tta
arrg
ge
ett ffo
orr C
Ca
all2
2 c
ca
alliib
brra
attiio
on
n
d
de
ep
pe
en
nd
diin
ng
g o
on
n tth
he
e tty
yp
pe
e o
off a
as
ss
sa
ay
y b
be
eiin
ng
g p
pe
errffo
orrm
me
ed
d.. IIff y
yo
ou
u a
arre
e u
us
siin
ng
g B
Biio
o--P
Plle
ex
x
M
Ma
an
na
ag
ge
err 5
5..0
0,, tth
he
e c
ca
alliib
brra
attiio
on
n iis
s d
do
on
ne
e o
on
n llo
ow
w R
RP
P1
1 tta
arrg
ge
ett.. S
Se
elle
ec
cttiio
on
n o
off h
hiig
gh
h R
RP
P1
1
tta
arrg
ge
ett iis
s d
do
on
ne
e iin
n tth
he
e v
va
alliid
da
attiio
on
n s
sc
crre
ee
en
n..
2.
Select IIn
ns
sttrru
um
me
en
ntt, then V
Va
alliid
da
attiio
on
n from the main menu and select F
Fllu
uiid
diic
cs
s in
the dialog box.
3.
Enter user name and control number. Select OK. The following dialog box
will then appear (Figure 6):
Fig. 6. Fluidics validation dialog.
4.
Add 5 drops each of fluidics beads 1 and 2 to the designated wells on the
MCV plate.
5.
Select the E
Ejje
ec
ctt button in the dialog box to eject the plate holder.
6.
Place the MCV plate in the microplate platform.
7.
Select O
OK
K to begin the fluidics validation procedure.
8.
When the procedure has been completed, results will appear in a dialog box,
as shown in Figure 7:
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