Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits - Manual (Page 27)

Acquire Data
1. Shake the assay plate at 900 rpm for 30 sec immediately before
acquiring data. Failure to do so will result in increased read time due
to bead settling.
2. Visually inspect the plate and ensure that the assay wells are filled with
buffer prior to placing the plate in the Bio-Plex microplate platform.
3. Slowly remove the sealing tape and any plate cover before placing
the plate in the reader.
4. Select the appropriate protocol (.pbx) file.
5. Select Start and save the report (.rbx) file.
6. Begin data acquisition.
NOTE: If acquiring data from more than one plate, empty the waste
bottle and refill the sheath bottle after each plate (if HTF not present).
Select Wash Between Plates and follow the instructions for
fluidics maintenance. Then repeat the Prepare Protocol and
Acquire Data steps. We recommend using the Wash Between
Plates command after every plate run to reduce the possibility of
clogging the instrument.
7. When data acquisition is complete, select Shut Down and follow
the instructions.
Reacquire Data
It is possible to acquire data from a well or plate a second time using the
Rerun/Recovery mode located below Start in Step 7 (Run Protocol). For
details, refer to the Bio-Plex Manager instruction manual.
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