Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits - Manual (Page 32)

Reaquire Data
It is possible to acquire data from a well or plate a second time using the
Rerun/Recovery mode located below Start in Step 7 (Run Protocol). For
details, refer to the Bio-Plex Manager instruction manual.
Bio-Plex Manager Software Version 4.0
Bio-Plex Manager version 4.0 does not include the 25-bead map. Hence,
the 100-bead map is used. Follow the instructions above for Bio-Plex
Manager version 4.1 and 4.1.1 for preparing and calibrating the system.
Be careful to calibrate on Low RP1 and in Step 7 (Run Protocol) under
Advanced Settings, set the DD gates to 5,000 (low) and 32,000 (high).
Acquire Data
1. Shake the assay plate at 1,100 rpm for 30 sec immediately before
acquiring data. Failure to do so will result in increased data
acquisition time due to bead settling.
2. Visually inspect the plate and ensure that the assay wells are filled with
buffer prior to placing the plate in the Bio-Plex microplate platform.
3. Slowly remove the sealing tape and any plate cover before placing
the plate in the reader.
4. Select Step 7 (Run Protocol):
a) Choose 100 beads per region.
b) In Advanced Settings, set the Bead Map to 100 region.
c) In Advanced Settings, set the sample size to 50 µl.
d) In Advanced Settings, confirm that the DD gates are set to 5,000
(low) and 32,000 (high).
NOTE: These settings are already selected in preset downloaded
protocols. If creating a new protocol, manually change the default
setting to reset the gates to 5,000 (low) and 32,000 (high).
e) Select Start and save the report (.rbx) file. Then follow the
instructions for data acquisition.
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