Bio-Rad Nonmagnetic Beads and Related Reagents User Manual
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Microtiter Plate Shaker
MTS 2/4 shaker for 2 or 4 microplates, IKA catalog #3208000
Sonicator
Branson ultrasonic cleaner model 1510-DTH, VWR catalog #21812-175
Cell Counter
Z2 Coulter Counter, Beckman Coulter Model Z2, catalog #6605700
Protein Assay
Microcentrifuge model 5415C, VWR catalog #20901-051
Microtiter Plate Shaker
Bio-Rad DC™ protein assay kit I (bovine gamma globulin standard),
Bio-Rad catalog #500-0111
Bio-Rad DC protein assay kit II (BSA standard), Bio-Rad catalog #500-
0112
Buffer Exchange Columns
Micro Bio-Spin™ 6 Tris chromatography columns, Bio-Rad catalog #732-
6221, 732-6222, or 732-6225
Chemicals
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC), Pierce catalog
#22980 N-hydroxysulfosuccinimide (S-NHS), Pierce catalog #24510
Reagent Reservoir
VistaLab catalog #3054-2002
VistaLab catalog #3054-1004
Other Materials
Antibody specific for coupled protein (labeled with R-phycoerythrin or
biotin), streptavidin-R-PE, pipets and pipet tips, aluminum foil, small weigh
boats, microcentrifuge tubes, 96-well flat-bottom plates, 15 ml conical
tubes (for large scale coupling).