Bio-Rad Nonmagnetic Beads and Related Reagents - Manual (Page 30)

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Section 11
Bio-Plex Protein Array
System Operation
System Preparation
Recommendations for reading the coupled beads on the Bio-Plex
suspension array system are listed below. Alternatively, refer to the Bio-
Plex Manager™ software user guide.
1. Turn on the Bio-Plex array reader and microplate platform (and high-
through put fluidics system if present). Allow the system to warm up
for 30 min.
Note: If the system is left idle for 4 hr, the lasers will automatically turn
off. A 30 min warm-up period will again be required prior to reading
an assay. Select Warm up from the tool bar and wait for the optics to
reach operational temperature.
2. Select Start up from the tool bar and follow the instructions shown on
the screen to prepare the reader.
Note: If the waste reservior is overfilled, the fluidics system may back
up and the assay signal will be lost. The sheath reservoir contains
enough fluid for approximately two 96-well plates. If the sheath fluid
level falls below the Sheath output tubing on the bottle, the assay
reading cannot be completed.
3. Select Calibrate from the toolbar and follow the instructions on the
screen. When entering the target values be sure to use the Low RP1
target value for the CAL2 calibration. Daily calibration is
recommended prior to reading the first assay.
Note: Be sure to calibrate the reader using the Low RP1 target value
printed on the label of the CAL2 beads.
4. Open a new protocol by selecting File then New from the main menu.
Locate the Protocol Settings steps on the left side of the protocol view.
5. Select Step 1 and enter any relevant information about the coupling.