Bio-Rad BioLogic LP System - Manual (Page 43)

Table 6. (continued)
Manual Mode Operation: Pump
Reference Description
Start/Stop Screen: The first softkey in the Main Menu screen
allows you to start/stop the pump using its currently set flow
rate. When the pump is running, its status light is ON.
•
Pressing Stop stops the pump.
• Pressing Purge increases pump speed to maximum. Refer
to the Purge screen.
• Pressing Flow allows you to set the flow rate. Refer to the
Flow screen.
• Pressing Buffer allows you to specify the buffer. Refer to
the Buffer screen
Purge Screen: Selecting Purge from the Main Menu screen
sets the flow rate to maximum and runs the pump. The Purge
screen is shown at left. During purge, the pump’s status LED
flashes. You then have the following choices:
•
Stop: Stops the pump.
• Run: Returns to the normal set flow rate and continues
operation. The status LED changes to ON.
• Buffer: Allows you to change the buffer. Refer to the
Buffer screen.
Flow Screen: To change the flow rate, use the keypad to enter
the desired flow rate and then press OK. To change the direc-
tion of the flow, press the Forward softkey. Forward is clock-
wise, and Reverse is counter-clockwise. Note: The arrow in
the display indicates the direction of flow.
In addition, the Flow screen’s menu allows you to calibrate the
pump, as described below. The system uses the flow rate to
determine fraction size and for gradient control when measuring
by volume rather than time. The system calculates the flow rate
by multiplying the pump head speed (rpm) by a flow rate factor
which is determined by calibrating the pump, as discussed
below. Note: You must select a tubing size or calibrate before
running a gradient method.
Calibrate: Values from the calibration are used by the system
to determine flow rate. Calibration is based on the tubing size
installed in the pump head. Calibration begins with the selec-
tion of a standard tubing size or non-standard (Other) tubing
sizes.
Note: Before calibrating, check the adjustment of the platen
pressure screw on the pump. Refer to section 4.2.
36
System Operation
M1
M2
Method: <<name>> Manual Operation
–> Flow: 1.00 ml/min UV: 0.0111 AU
Buffer: A Cond: 0.000 mS
STOP RUN BUFFER
Method: <<name>> Manual Operation
–> Flow: 1.00 ml/min UV: 0.0111 AU
Buffer: A Cond: 0.000 mS
CALIBRATE FORWARD CANCEL OK
M3
Method: <<name>> Manual Operation
Current Tubing Diameter: 3.2 mm
Press "PUMP" to CANCEL
0.8 mm 1.6 mm 3.2 mm OTHER
M3a
Method: <<name>> Manual Operation
–> Flow: 1.00 ml/min UV: 0.0111 AU
Buffer: A Cond: 0.000 mS
STOP PURGE FLOW BUFFER
M1 M2 M3 M4
M1 M1 M4
M3a M1
M3b M3d
M1
MP
M1 MP
M1
M3
MP
(REVERSE)