Luminex 100 IS User Manual Addendum Version 2.3 - Manual (Page 5)

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MAP Technology Addendum to User Manual Version 2.3
PN 89-00002-00-140 Rev. A 3
Storing the Analyzer
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To prepare the system for storage:
1. Run a Sanitize command with 10% to 20% household bleach
solution.
2. Run a Sanitize command with distilled water.
3. Run four Wash commands with distilled water.
4. Remove the sample probe from the instrument, flush with
distilled water from the narrow end out through the larger end,
replace it in the sample arm and wrap the end with Parafilm.
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To prepare the system for first use after it has been in storage:
1. Turn on the Luminex 100 and XY Platform and watch for the
following indications that the instruments are responding
correctly:
• The light above the sample probe on the Luminex 100 and the
light next to the door of the XY platform are lit.
• The compressor starts in the Luminex 100. It is a low
rumbling sound.
• Place your hand behind the Luminex 100 to feel air coming
out of the rear fan. Place your hand on the left side of the XY
to feel air coming out of the XY.
• Observe movement of the syringe inside the front middle door
of the Luminex 100 shortly after the instrument is powered on.
2. Turn on the PC and start up the IS software.
3. Complete a Warmup command, which will take 30 minutes.
4. Remove the Parafilm from the end of the sample probe.
5. After Warmup is complete, run three Backflush commands, three
Drain commands, two Alcohol Flush commands, and three Wash
commands with distilled water. Make sure that the sheath bottle
or Luminex SD has a sufficient amount of sheath fluid and that
the waste container is empty. Verify that the pressure during each
of the maintenance commands reads between 6 and 9 psi in the
System Monitor.
Sample Volume/
Sample Dilution
For optimal sampling efficiency and to reduce matrix effects,
concentrated biological fluids such as serum or plasma should be
diluted at least 1:5.
To minimize the chance of acquiring air, be sure that the sample
acquisition volume is at least 25 µL less than the total volume in the
well.