Luminex 100 IS User Manual Addendum Version 2.3 - Manual (Page 7)

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MAP Technology Addendum to User Manual Version 2.3
PN 89-00002-00-140 Rev. A 5
Sheath Delivery
System Setup
When adjusting the Luminex 100 regulator, turn it clockwise as far
as it will go to the fully closed position, then turn it back 1/2 of a
turn. Be careful not to force the regulator past the fully closed
position.
Replacing the
Sheath Delivery
System with Sheath
Bottles
You may need to replace the Sheath Delivery (SD) system with
sheath bottles for maintenance or troubleshooting, as directed by
Luminex Technical Support.
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To replace the SD system with sheath bottles:
1. Power off and disconnect the SD system from the Luminex 100.
2. Turn the black regulator screw inside the center door of the
Luminex 100 one full turn counter-clockwise.
3. Attach the sheath and waste bottle containers. Make sure there is
sheath fluid in the bottle (to the fill line) and the sheath cap is
tightened.
4. Prime the system.
5. As the system is priming, adjust the black regulator screw inside
the center door of the Luminex 100. Turn clockwise to increase
or counterclockwise to decrease, until the sheath pressure value
is within +/- 0.1 psi of the normal level of your instrument.
Please visually monitor the pressure gauge on the Run Batch
tab. Make sure pressure is centered within green tolerance
range.
6. When the prime function is complete, loosen the sheath bottle
cap to release pressure.
7. Tighten sheath cap and prime the system once again.
8. Make sure that the sheath and air pressure values increase to the
normal level once again.
Probe Height
Adjustment
This information supplements the information in the IS 2.3 User
Manual.
Be sure the plate you are using is completely flat – warped plates can
lead to incorrect probe height adjustment.
In a 96-well microtiter plate where overall height is no more than
0.75 inches (19 mm), place the appropriate alignment tool in the
plate: