Acquisition problems, Verification problems, Verification problems continued – Luminex FLEXMAP 3D User Manual User Manual
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Symptom
Possible cause
Solution
The waste line was moved during
instrument operation, resulting in an
unstable flow rate.
Stabilize the waste line during instrument
operation. See the section titled
“
There is a problem internal to the
instrument.
Review the log of calibration reports.
Check for dramatic changes in
temperature, sheath pressure, or voltage. If
any of these are present, contact Technical
Support.
Acquisition Problems
TABLE 8.
Verification Problems
Symptom
Possible cause
Solution
Acquisition fails or is slow.
The air pressure is out of range.
See the section titled “
”.
The sample probe height is incorrect.
Perform an automatic sample probe height
adjustment.
The sample probe is clogged.
See the section titled “
”.
The sheath or waste lines are not fully
connected.
Disconnect and reconnect the lines; you
should hear them click.
The calibration microspheres have expired. Replace old microspheres with an
unexpired lot.
The wrong wells are selected for the
calibration microspheres.
Ensure that the correct wells are selected
in the software.
The wrong lot number or target values are
entered in the appropriate page of the
software UI.
Check the lot number and target values to
make sure they are correct.
Air is present in the instrument.
Verify the sample probe height. Use the
software to run a prime command three
times, an alcohol flush command twice,
then a wash command three times with
deionized water.
The acquisition volume is set too high.
Set the acquisition volume to at least 25μL
less than the actual volume in your wells.
This enables the probe to acquire sample
more efficiently with less chance of
incorporating air in the sample.
The Luminex
®
xMAP
®
microspheres are
not fully suspended.
Gently vortex the microtiter plate or
resuspend beads with a multichannel
pipettor to ensure that the microspheres
are present in the solution.
TABLE 7.
Verification Problems Continued
For research use only. Not for use in diagnostic procedures.
Troubleshooting
49