Eppendorf epMotion 5075 - Manual (Page 56)

Dispensing liquids
epMotion
® 5075
English (EN)
56
6.2 Dispensing modes
The epMotion can be used to dispense liquids in the Pipette and Multidispense modes.
6.2.1 Dispensing mode: Pipette
In the Pipette mode, a defined volume of liquid is aspirated and fully dispensed.
Dispensing errors are smaller than in the Multidispense (see Dispensing tool errors on p. 115) mode.
The epMotion executes a blow-out in the Pipette mode. Residual liquid from the pipette tip will be
dispensed in the destination vessel. The blow-out is part of the dispensing volume. The blow-out can be set
in the software (refer to the epMotion software operating manual).
In the Pipette mode, the smallest dispensing volume for water is 1 μL.
6.2.2 Dispensing mode: Multidispense
In the Multidispense mode, liquid is aspirated and dispensed in the destination vessels in defined partial
quantities.
Dispensing errors are larger than in the Pipette (see Dispensing tool errors on p. 115) mode.
The epMotion carries out a remaining stroke in the Multidispense mode. Residual liquid from the pipette tip
will be dispensed in the waste vessel or source vessel. The residual liquid is not part of the dispensing
volume.
In the Multidispense mode, the smallest dispensing volume for water is 3 μL.
6.2.2.1 Aspiration volume at Multidispense
To create the same physical conditions for all dispensing steps, more liquid must be aspirated in the
Multidispense mode than the sum of the dispensing volumes. This is the result of the reverse stroke and the
remaining stroke.
Reverse stroke
• The reverse stroke occurs after liquid aspiration.
Part of the aspirated volume is immediately returned to the source vessel.
The reverse stroke is the same size for all liquids.
Remaining stroke
• After the reverse stroke, there is more liquid in the pipette tip than the amount required for the
dispensing steps. This additional aspiration volume will be dispensed as a remaining stroke after
dispensing.
The additional aspiration volume will be dispensed to the source vessel if the tips are not replaced
before each liquid aspiration.
The additional aspiration volume will be dispensed to the waste container if the tips are not replaced
before each liquid aspiration.