Bio-Rad Experion RNA Analysis Kits - Manual (Page 30)

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Fig . 4 .10 . Results and settings . Shown is the Results tab
for a single sample.
4.1.5 Results and Settings
The six results and settings tabs (Figure 4.10) present options for viewing, customizing, and analyzing
separation data (see Section 4.3, Using Results and Settings to View and Annotate Data).
4.2 Changing the Fluorescence Intensity Scale
To display more or less detail in the electropherogram traces and lanes in the virtual gel, use the options
available under the Graph menu. These options determine the y-axis scale (fluorescence intensity) of
electropherograms and define the relative scale intensity of the lanes in the virtual gel (Figure 4.11):
n
Scale To Selected — uses the fluorescence value of the tallest peak in a selected
electropherogram to set the scale of the y-axis for all samples. Use this option when
comparing (overlaying) a well with a high-intensity peak and one with a less intense peak
n
Scale To Global — uses the fluorescence value of the tallest peak from all the
electropherograms to set the scale of the y-axis for all samples. Use this option to create a
virtual gel similar to an agarose gel
n
Scale To Local (default) — uses the fluorescence value of the tallest peak in each
electropherogram to set the scale of the y-axis for that electropherogram: each sample is
scaled separately. This is the best way to view electropherograms and sample details
n
Scale To Selected Peak — uses the fluorescence value of the selected peak to set the
scale of the y-axis for all samples
Fig . 4 .11 . Options for modifying the fluorescence intensity scale . Examples of how each option affects the display of a virtual gel are shown.
Scale To Global Scale To Selected Peak Scale To Local Scale To Selected
Experion Automated Electrophoresis System