Bio-Rad Experion RNA Analysis Kits - Manual (Page 7)

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Table 1.1. Experion RNA analysis kit specifications.
Experion RNA StdSens Experion RNA HighSens
Number of samples 12 11
Sample volume 1 µl 1 µl
Quantitation range:
Total RNA 25–500 ng/µl 200–5,000 pg/µl
mRNA 25–250 ng/µl —
Limit of detection (total RNA) 5 ng/µl 100 pg/µl
Maximum salt concentration TE buffer (10 mM Tris, 1 mM EDTA) DEPC-treated water
1.2 Kit Components
Table 1.2. Components of the Experion RNA analysis kits.
Item Description Volume (per Vial) 10-Chip Kit
RNA StdSens or Microfluidic chips used for RNA separation 10 chips
RNA HighSens chip
Cleaning chip
Chip used for cleaning electrodes 2 chips
RNA gel Proprietary polymeric sieving matrix 1,250 µl 1 vial
RNA StdSens or Proprietary fluorescent dye 20 µl 1 vial
RNA HighSens stain
RNA StdSens or
Buffer for sample preparation; contains lower marker 900 µl 1 vial
RNA HighSens loading buffer for alignment of samples to the RNA ladder
RNA ladder Standard containing 8 RNA fragments of 200–6,000 nt 20 µl 1 vial
RNA sensitivity enhancer Proprietary reagent to improve sensitivity 100 µl 1 vial
(RNA HighSens kit only)
Spin filters
Used for filtering reagents during sample preparation 3 filters
1.3 Storage Conditions
Table 1.3. Storage conditions.
Item Storage Shelf Life
Experion reagents 4ºC See expiration date on packaging
Experion chips Ambient See expiration date on packaging
Experion RNA ladder –70ºC See expiration date on packaging
Filtered gel (G) 4ºC 1 month from filtration; after 1 month, refilter
Experion RNA StdSens and HighSens Analysis Kits