Bio-Rad Experion RNA Analysis Kits - Manual (Page 54)

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Once separation occurs, the data are converted into a densitometric, gel-like image, or virtual gel (Figure
A.3). Each lane in the virtual gel corresponds to a different sample. The sample peaks and lower marker
seen in the electropherogram also appear in the virtual gel.
Fig . A .3 . Virtual gel generated by Experion RNA StdSens analysis of mRNA (left) and eukaryotic total RNA (right) .
Shown are separations of the RNA ladder (L) and 12 samples on a single chip. Pink triangles mark the position of the lower marker.
Lower marker
Once separation is complete, the software subtracts background noise, removes spikes, identifies and
integrates peaks, and assigns their sizes and concentrations. The results of data analysis are tabulated
and presented in a Results table at the end of analysis (Figures A.4 and A.5). The types of data available
include the following:
n
Concentration of mRNA or total RNA (in ng/μl or pg/μl, depending on the assay)
n
rRNA contamination (mRNA assays only)
n
28S/18S ratio (eukaryotic total RNA assays) or 23S/16S ratio (prokaryotic total RNA assays)
n
RNA quality indicator (RQI, eukaryotic total RNA assays)
Fig . A .4 . Results tab generated by
Experion RNA StdSens analysis of
eukaryotic total RNA . Shown is the
Results tab for a single sample.
Experion Automated Electrophoresis System