Jenway Genova Nano German - Manual (Page 69)

14.6 260 / 280
The 260/280 operating menu enables the purity and
concentration of DNA to be estimated according to the
following equations:-
Purity Ratio = Abs@260nm / Abs@280nm
Conc (μg/ml) = (Abs@260nm x 62.9) - (Abs@280nm x 36.0)
The 260/280 settings menu allows the user to adjust
measurement specific parameters such as the dilution factor
and resolution but the concentration units are limited to
µg/ml and mg/ml only. Other method settings, such as the
inclusion of a reference wavelength and the concentration
equation and factors can be altered if required, but the
260/280 method settings are locked and any changes
will be lost after exiting the mode. See Section 13 for full
instructions on altering the 260/280 method options and
settings.
14.7 260 / 230
The 260/230 operating menu enables the purity and
concentration of DNA to be estimated according to the
following equations:-
Purity Ratio = Abs@260nm / Abs@230nm
Conc (μg/ml) = (Abs@260nm x 49.1) - (Abs@230nm x 3.48)
The 260/230 settings menu allows the user to adjust
measurement specific parameters such as the dilution factor
and resolution but the concentration units are limited to
µg/ml and mg/ml only. Other method settings, such as the
inclusion of a reference wavelength and the concentration
equation and factors can be altered if required, but the
260/280 method settings are locked and any changes
will be lost after exiting the mode. See Section 13 for full
instructions on altering the 260/230 method options and
settings.
14.8 VARIABLE RATIO
The Variable Ratio operating menu enables the purity and
concentration of DNA to be estimated according to the
following equations:-
Purity Ratio = Abs@Xnm / Abs@Ynm
Conc (μg/ml) = (Abs@Xnm x f1) - (Abs@Ynm x f2)
Where X, Y, f1 and f2 are defined by the user.
Operating Menu
Operating Menu
Operating Menu
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