Iris Sample Processing Statspin® MP Multipurpose Centrifuge - Manual (Page 14)

10
Section
5
Specimen Processing
Venous Blood for Coagulation and Chemistry
PlasmaRotor ® (RD01) consumable provides a means for the rapid separation of plasma from
anticoagulation whole blood, up to 3 mL. To prepare plasma for chemistry testing use the
PlasmaRotor cycle and for Coagulation testing using the Coag Plasma cycle.
Preparation of Urine Sediment for Microscopic Examination
The StatSpin MP quickly prepares urine sediment for microscopic examination. This is accomplished
with the precalibrated urine tubes. (Product Number TU15)
Procedure
1. Add fresh urine to a Urine tube (Product No. TU15) fill to the top mark (representing 1.5 mL).
2. Cap the tube using the attached stopper and centrifuge in the Tube Rotor, RT12.
3. Balance the rotor either with another sample or with a water-filled tube. This balance tube does
not have to be exact.
4. Select the “Urine” setting.
5. When the cycle is complete the cover will release. Remove the tube from the rotor and remove
the stopper.
6. Invert the tube to drain fluid to the lower mark. (The surface tension will retain 0.1 mL.)
7. Recap the tube and re-suspend the sediment at the bottom of the tube by holding the tube with
the index finger and thumb and “flicking” the tube with the opposite hand.
8. After sediment has been re-suspended, apply one drop to a microscope slide, apply a cover slip
and read following the protocol used in your laboratory.
Lipemia Clearing with LipoClear ®
StatSpin MP can be used to centrifuge samples treated with LipoClear (LC10, LC40, LC15). LipoClear
is a non-toxic, non-carcinogenic, lipemic sample clearing reagent, pre-filled in micro centrifuge tubes.
The kit is available for 0.5 mL and 1.5 mL sample sizes (see table below). After sample is added,
mixed and allowed to stand for 5 minutes, the tubes are spun on the LipoClear cycle in the RT12 Tube
Rotor. See LipoClear Product Insert Sheet for details.
Product No.
Sample Size Packaged As
LC10 0.5 mL 10 tubes/ pack
LC40 0.5 mL 40 tubes / pack
LC15 1.5 mL 40 tubes / pack
Determination of Packed Red Cell Volume or Microhematocrit
Both glass and plastic micro-capillary tubes are available. Product Number HT9H(glass) &
HP8H(plastic) have been pretreated with heparin and should be used for capillary blood. They
should be stored in a cool dry place. Product Number HT9U(glass) & HP8U(plastic) are untreated
and used for venipuncture (anticoagulated) samples.
For glass tubes only: if the vial of tubes is new, unscrew the top, remove and discard the foam
cushion and reattach the top. Now a single tube at a time can be shaken from the vial through the
small hole in the center of the cover.
Fill to here
Empty to here