Iris Sample Processing Statspin® MP Multipurpose Centrifuge - Manual (Page 15)

11
Procedure
1a. Capillary ("fingerstick") blood - prepare a skin site and lance. Use heparinized tubes, Product
Number HT9H or HP8H.
or
1b. Venous blood - take well-mixed anticoagulated blood from a syringe or a vacuum blood
collection tube. Use untreated tubes, Product Number HT9U or HP8U.
2. Hold the micro-capillary tube by the end with the color-coded band. (See Figures, page 12)
3. Fill to the color-coded band. Remove from sample and tilt the banded end downward until the
blood moves half-way between the band and the end of the tube.
4. Hold the tube in a horizontal position and push the dry (banded) end of the tube fully into the
vertically held sealing compound. Twist and remove.
5. Using a laboratory tissue wipe off any blood that is forced from the other end.
6. Put the tube, sealed end towards the outer rim, in any of the twelve positions on the
Hematocrit Rotor, RH12. This rotor need not be balanced. Screw cover in place.
7. Holding the rotor by the black "cover knob", attach the rotor to the rotor-holder.
I MPORTANT : Always hold hematocrit rotor by the black knob on the rotor cover, when pressing
it firmly in a downward motion onto the rotor-holder and when removing the rotor from the
centrifuge. Pressing on the outer edges of the Hematocrit Rotor, RH12, may result in damage
to the rotor.
8. Centrifuge the Hematocrit Rotor.
9. After the rotor stops, remove the rotor. To read hematocrit, place the rotor into the middle of
the illuminated, digital reader. Follow directions printed on the reader.
10. Spun tubes inside the Hematocrit rotor can also be read with the circular reader, HR4C as well
as removed from the rotor and read with the card-style reader, HR05.
Quality Control
1.
The Quality Control procedures established for your laboratory should be followed.
2.
To verify the adequacy of cell packing, on a daily basis, select one or more tubes,
(preferably with a hematocrit over 50), centrifuge and read. Spin these tubes a second time.
The difference between the initial reading and the second reading should be 1 percent or
less.
Normal Values
The following tables represent commonly accepted hematocrit values:
Children
Age %
Birth 44 - 64
14 - 90 days 35 - 49
6 months - 1 year 30 - 40
4 - 10 years 31 - 43
Adults
Gender Mean % Range (2 s.d.)
Males 47 40 - 54
Females 42 37 - 47
Animals
Species %
Canine 37 - 55
Feline 24 - 45
Equine 32 - 52
Bovine 32 - 38
Porcine 32 – 50
Ovine 24 – 45