Luminex 100 IS Version 2.3 - Manual (Page 181)

PN 89-00002-00-072 Rev. C A - 3
x
MAP Technology Luminex xMAP microsphere set
Luminex xMAP
microsphere set
Luminex multi-analyte microspheres containing a unique mixture of
two distinctly colored fluorochromes to distinguish them from other
multi-analyte microspheres.
laser
Light Amplification by Stimulated Emission of Radiation (laser).
This highly purified source of light is an efficient way to excite
fluorochrome electrons.
microparticle
A solid substance with a diameter in the micrometer range. Often
used as a synonym for a microsphere.
microspheres
Polystyrene spheres with a diameter in the micrometer range. Also
called beads.
multi-analyte
Several assays or tests performed simultaneously in the same
reaction container.
multi-batch
A set of batches to be processed consecutively.
photobleaching
The process in which light absorption converts the fluorochromes
inside the beads into different fluorescent or nonfluorescent
compounds. Photobleaching prevents beads from being properly
classified.
PMT
Photomultiplier tube, measures the excitation emission intensity of
the reporter dye bound to the surface of the xMAP microspheres.
qualitative
Pertaining to calculations that determine the absence or presence of
an analyte.
quantitative
Pertaining to calculations that determine the precise numerical
measurement of an analyte.
reporter
A molecule (or combination of molecules) with a specific range of
excitation and emission wavelengths that is used to identify or
quantify an analyte. Examples of acceptable reporters for the
Luminex 100 IS are Phycoerythrin and Alexa 532.
reporter channel (RP or RP1)
A specific range of wavelengths that includes the emission
wavelength of a designated reporter molecule.
RP1
Refers to the dyes bound to the surface of the xMAP microsphere.
Also see reporter channel.
RP
See reporter channel.