Bio-Rad Bio-Plex Manager™ MP Software Upgrade - Manual (Page 28)

3 | Running Protocols
26 | Bio-Plex Manager MP Software
Plate Handling Guidelines
Before starting the run, note the following plate handling guidelines and warnings:
 Protect your assay microspheres from light. Once photobleached, the
beads are no longer usable. You must care for the microspheres properly
to maintain your product warranty.
 When using a filter plate and wrapping the plate, avoid touching the
bottom of the wells. The sample may wick or leak from the well bottom.
Should this occur, it can cause problems when reading the plate.
 Make sure you have added at least 125 µl of sample to all the wells
specified in your plate template before starting a protocol run. If the array
reader attempts to draw sample from an empty well, air is sucked into the
sample loop and injected into the flow chamber. When this happens,
bubbles form in the cuvette and interfere with the analysis. Should this
occur, perform a Clear Bubbles procedure (see Clear Bubbles Routine on
page 77 ), then rerun the protocol.
 Shake the microplate on a plate shaker for 30 seconds prior to performing
a reading.
For some experiments the temperature of the plate must be maintained at a
particular temperature. See Using the Plate Heater on page 28 for more information.