Olympus IX2-DSU BX-DSU - Manual (Page 15)

SYSTEM OUTLINE
/ Principle
I. System Outline
I . 1 - 1 Page
1 SYSTEM OUTLINE
Attaching this system to a microscope, it comprises a confocal microscope system without laser, but with use of
a white light source. It acquires a high contrast image through resolution enhanced in light axis direction very
rapidly.
In addition, Olympus motorized microscopes IX81, BX61WI, BX61 or BX62 and their related motorized units
may be controlled through a 3rd party software, which is designed for observation by CCD camera.
And the combination of such the software and the microscope may enable 3 dimensional observation, time-
lapse observation and so on.
The software controls changeover of confocal/non-confocal mode, ND filter synchronized to confocal or non-
confocal mode and filter cube inside the disk box in conjunction with motorized focusing control of motorized
microscope and thus, by changing wavelength through Z-drive of focusing control, it acquires images for myriad
observation.
1-1 Principle
Light emitted from light source of mercury or xenon
reflects at fluorescent mirror and enters rotary disk
in light path. The rotary disk has a pattern of linear
lines that provides the effect equivalent to pin hole
by high speed rotation. Light that passes through
the pattern further passes through objective lens
and goes to the specimen. Fluorescence emitted
from the specimen passes through the objective
lens again and forms an image on the rotary disk.
Among image formed, a portion of light that is in
focus will pass through the disk and, CCD camera
captures the image and the specimen image will be
displayed on the monitor.
Light
source
ND Filter
Objective lens
Imaging lens
Imaging lens
Rotary disk
CCD Camera
Monitor
Specimen
Light
illuminator
Fluorescent
Mirror unit
Disk box
Camera adapter
Illumination
tube