2 summary of observation procedures – Olympus IX2-DSU BX-DSU User Manual

Page 23

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SUMMARY OF OBSERVATION PROCEDURES

II.

DSU

II .

2 - 1

Page

2 SUMMARY OF OBSERVATION

PROCEDURES

This section describes procedures for fluorescent observation by visual method followed by camera observation

by confocal mode.

Preparation
•Install fluorescent mirror unit and objective lens applicable to observation method.
•Do centering of mercury lamp or xenon lamp.
•Bring camera in focus.

Procedures

Where

Ref.

Turn power switch of each system to ON position

and do initialization.

..........................

Power switch of each
system

3-5

Turn power switch of lamp to ON position and wait

until arc is stabilized. (5 to 10 minutes).

..........................

Main switch of lamp power

3-5

Set specimen on stage.

..........................

Lateral/Longitudinal feed
handle

-

Turn turret of front side and insert visual mirror unit

that fits to specimen into light path.

..........................

Visual mirror unit (front
side)

3-7

Turn turret of rear side and insert No.1 reference

mirror into light path

..........................

Camera mirror unit (rear
side) change button

3-10

Displace disk from light path.

..........................

Disk IN/OUT button

3-8

Put objective lens in light path and open shutter to

bring the specimen in focus.

..........................

Objective lens change
button, revolving
nosepiece, shutter
open/close knob or Electric
shutter or focusing handle
or coarse/fine handle

3-6
3-9

Adjust light intensity with use of

ND filter to put in or remove

from light path.

Filter change button

3-11

Adjust entire field of view to be uniformly bright.

..........................

Collector lens focusing
knob

3-12

Adjust field iris diaphragm.

Field iris diaphragm lever

3-12

Visual observation

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