Luminex 100 IS User Manual, Version 2.3 - Manual (Page 107)

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MAP Technology Using Luminex 100 IS 2.3 Software
PN 89-00002-00-071 Rev. C 5 - 65
3. Select the item that uses the dilution factor that you are setting.
You cannot change any item that is grayed out or locked into the
command list.
Figure 5-34. Luminex Batch Setup for Dilution
4. To change the dilution factor, click the Dil. Factor field. The
white background indicates that you can enter text. Enter each
dilution factor as a decimal, not as a ratio.
5. Click Finish. The system saves the information you entered.
Create a New
Advanced Batch
Use New Advanced Batch to acquire data without creating a
template. It writes raw data results to a simple csv file format. You
can define parameters for samples, gates, regions, events, on-plate
and off-plate commands. This feature does not store the results in the
Luminex System database or allow you to perform data analysis on
acquired batches. The Luminex analyzer uses only the 100 region
bead map. However, in the Acquisition Detail tab, you can view the
data for 25, 50, 64, and 100 regions and save the data to a csv file.
Use plates with wells that will hold at least 185 µL (the extra 25 µL
from the sample, plus an extra 160 µL that is dispensed back into the
well following acquisition).