Luminex 100 IS User Manual, Version 2.3 - Manual (Page 108)

Luminex 100 IS User Manual Version 2.3
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MAP Technology
5 - 66 PN 89-00002-00-071 Rev. C
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To create a New Advanced Batch:
1. Open the Acq. Detail tab.
2. On the Acquisition Detail toolbar, click New Advanced Batch.
The Options Dialog Box opens with the General tab displayed.
See Figure 5-35.
.
Figure 5-35. Options Dialog Box—General Tab
3. Enter the Name, Description, and Operator information.
4. Edit the following information as desired:
• Sample Size - use values from 20 to 200 µL. To avoid air
uptake, we recommend that your sample well contains at least
25 µL in addition to the sample size.
• DD Gate - use values within the range of 0 to 32767.
• Timeout - use values of 0 to 400, where 0 = no timeout.
5. Click the arrow down next to 100 regions to select the desired
bead map you want to view. The available bead maps are 25, 50,
64, and 100 (default) regions. Select whether you want the Bead
Events results to be displayed as Per Bead or Total Beads. If
you select Total Beads, enter the number of total beads in the text
box.
• Select Per Bead to continue analyzing until each bead set has
met at least the minimum events as determined on the Bead
Set tab.
• Select Total Beads to continue analyzing until the selected
beads meet total beads value. Use Total Beads when you are
not using all of your selected bead sets in each well. Set the
total to desired high value.