Luminex 100 IS User Manual, Version 2.3 - Manual (Page 56)

Luminex 100 IS User Manual Version 2.3
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MAP Technology
5 - 14 PN 89-00002-00-071 Rev. C
The Batch Name and Description section displays the name of the
batch as well as its description.
The Batch Data area displays sample results. The left column shows
plate location and Sample ID description. The remaining columns
display selected bead sets for the assay. Each row represents the data
for each bead set from one well.
The Acquisition Detail Toolbar shown in Figure 5-12 provides
functions to acquire raw data without using templates. The toolbar
has additional buttons if you have Developer’s Workbench installed.
For more information about the commands on the Acquisition Detail
Toolbar, see the Command section, beginning on page 5-31.
Figure 5-12. Acquisition Detail Toolbar
The Histogram, in the lower left section of the Acquisition Detail
tab, defaults to display the Doublet Discriminator (DD) on the X axis
and Events on the Y axis. Doublets appear when two microspheres
stick together, creating undesired results. When you enable the gate,
two vertical red, dashed lines appear to represent gate positions
determined by the template or settings established within the New
Advanced Batch. After setting the gate, everything outside the gate is
ignored. You cannot change the gate position during batch
acquisition. You can change the gate positions before data
acquisition, after the system finishes acquiring data, or after pausing
the system. The change is visual. The data is still collected according
to the gate positions set in the assay template or New Advanced
Batch setup. The gate in effect when the system collects data
determines which values to use to obtain the result. Applying a gate
or changing a gate for existing data does not change your calculated
values. The gate positions, also located in the lower corner of the
histogram, are used while collecting data are the numerical values
selected in the template or the New Advanced Batch.
1. Replay Batch 6. Cancel All
2. New Advanced Batch 7. Eject/Retract
3. View Batch Data 8. Pause
4. Start Plate 9. Resume
5. Cancel Command
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