Accuracy and precision, Sensitivity, Capacity – Luminex 100 IS User Manual, Version 2.3 User Manual
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Luminex 100 IS User Manual Version 2.3
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MAP Technology
3 - 4
PN 89-00002-00-071 Rev. C
acquiring sample, running system calibrators, running system
controls, and warming up the instrument, the system resets the
four-hour internal clock.
Accuracy and Precision
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Sample uptake volume: ± 5%
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Classification of xMAP microspheres: > 80%
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Misclassification of xMAP microspheres:
≤ 2% - may vary by
xMAP microsphere product lines. Refer to the specific product
information sheet for further details.
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Temperature control: 0°C to + 2°C of target
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Internal sample carry over: < 0.9%
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Soluble background fluorescence emission at 575 nm
automatically subtracted from fluorescence intensity values
Sensitivity
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Detect 1000 fluorochromes phycoerythrin (PE) per xMAP
microsphere
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Reporter channel dynamic range: 3.5 decades of detection
Capacity
The specifications below reflect minimum capacity values:
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10 GB hard drive
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100 MB read/write CD ROM
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Analyze multiple 96-well plates per batch
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Analyze multiple assay templates per plate
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Distinguish a minimum of 1 to a maximum of 100 unique xMAP
microsphere sets in a single sample
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Detect and distinguish surface reporter fluorescence emissions at
575 nm on the surface of 1-100 unique xMAP microspheres sets
in a single sample
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Sample core: 15-20 µm core at 1 µL/sec. sample inject rate
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Maintain samples at a constant temperature from 35°C to 55°C
(95°F to 131°F)
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Automatic sampling from a 96-well plate
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Start sampling from any well position
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Sheath container and waste container hold enough volume to run
up to two 96-well plates between refills
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Microtiter plates with 96 wells must be compatible with the
Luminex XYP instrument plate holder. The following microtiter
plate types are compatible with the Luminex XYP instrument
plate holder: flatbottom, conical, round, filter bottom, half plates
[overall height no more than 0.75” (19 mm)], any color
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Microtiter plates with 96 wells must be compatible with
Luminex XYP instrument heater block temperature from 35°C to