Venous blood collection (venipuncture), Supplies, Procedure – Drucker Diagnostics QBC STAR Dry Hematology Analyzer User Manual

Page 82

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QBC Star System Operator’s/Service Manual

6000-300-000 E–2

venous Blood Collection (venipuncture)


The following procedure can be used as a guideline for venous blood collection. Please consult your
lab’s standard operating procedure as it may differ from the procedure described below.

Supplies

• Disposable gloves

• Tourniquet

• Alcohol pads

• Sterile gauze

• Bandage

• VACUTAINER® Brand (or other) evacuated blood collection system:

- Tubes containing EDTA anticoagulant (lavender top)

- Needles

- Needle holder/adapter

• Sharps container

• Marking pen

Procedure

1 Identify the patient by having him or her (or a guardian) state his full name.

2 Select the appropriate blood collection supplies. Establish the order of evacuated tubes if

multiple specimens are drawn.

3 Label all evacuated blood collection tubes with the patient’s name and the time and date the

specimen is drawn.

4 Position the patient with the elbow extended and the arm supported. Have the patient make

a fist, but avoid vigorous pumping or other hand exercise.

5 Apply the tourniquet about 3 - 4 inches above the venipuncture site. Do not stop the blood

flow for more than one minute before the blood is drawn. If necessary, release and reapply the
tourniquet.

6 Select the venipuncture site. The median antecubital and cephalic veins are most commonly

used.

7 Clean the venipuncture site with an alcohol pad, making one smooth, circular pass of the veni-

puncture site. Allow the skin to dry, to prevent hemolysis and to prevent the patient from having
a burning sensation when the needle is inserted. Do not touch the vein site after cleaning it.

8 Perform the venipuncture:

a Wearing gloves, gently grasp the patient’s arm near the venipuncture site, using the thumb

to draw the skin tight.

b With the needle bevel facing up, line up the needle with the vein. Penetrate the skin and

enter the vein at an angle of approximately 15 - 30 degrees. Holding the flange of the needle
adapter, push the evacuated tube forward, allowing the back end of the needle to puncture
the stopper to engage the vacuum.

6000-300-000 E-2

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